ObjectiveTo study the effects of different CO2 pressures on adhesive and invasive alility of MKN-28 gastric carcinoma cells.
MethodsWith an artificial pneumoperitoneum model in vitro, MKN-28 human gastric carcinoma cells were exposure to CO2 in 3 different pressure groups: 9mmHg, 15mmHg and control group. The adhesive ability of MKN-28 cells before and after exposed to CO2 was detected by CytoMatrixTM kit. The percentage of cells expressing E-cadherin and ICAM-1 among MKN-28 cells were counted by flowcytometry in vitro. All the MKN-28 gastric carcinoma cells were injected into abdominal cavity of nude mice(2×106cells/mouse) respectively. Five mice of each group were sacrificed 4 weeks later to record the number of tumor nodules in abdominal cavity.The remaining mice were kept to observe the survival time.
ResultsIn the 3 groups,all of the adhesive ability of MKN-28 cells (cd/m2). the percentage of cells expressing E-cadherin,and ICAM-1; the number of intra-abdominal tumor metastases. the survival time and the tumor metastasis in abdominal cavity of nude mice were no sigmificant difference call (P＞0.05).
ConclusionsWhen the pressure is below is below 15 mmHg and for less thqn 4 h, different CO2 pressures and different time intervals do not affect the adhesive and invasive ability of MKN-28 cells in the abdominal cavity,and not increase the chancws of tumonr metastasis.