Objective:To investigate the effect of enteral microecopharmaceutics nutrition(EMN) therapy on the inflammatory reaction and intestinal mucosal lesion in the early phase in severe acute pancreatitis(SAP) rats.
Methods:Sixty rats were divided randomly into control group(C group, n=20), traditional enteral nutrition group (EN group, n=20) and EMN group (n=20). The nutrition was given 8 h after modeling. Animals were killed at 12,24,48 and 72h after modeling, MIF level was measured in serum and the histopathological changes were checked. The expression of TLR4 mRNA in the small intestinal tissue was detected by reverse-transcription polymerase chain reaction (RT-PCR).
Results:(1)At 12 h after modeling, serum MIF level was increased higher in EN group and EMN group than C group(P＜0.01). At 72h, MIF level was decreased to varying degrees between three groups; EMN group was significantly lower than the EN group［(95.33±1.96) vs. (117.59±1.86),P＜0.05］. (2) On histopathologic examination of the small intestinal mucosa，no changes were observed in the C group wihle damage was seen in EN and EMN group, but the degree of damage was less severe in EMN group than EN group.(3)Compared with control group, TLR4 mRNA level markedly increased 12 h after modeling in EMN group and EN group(P＜0.01), and was significantly lower in EMN group than EN group after 72 h of modeling［(0.56±0.33)vs.(0.71±0.51),P＜0.01］.
Conclusions:EMN can be effectively regulate the levels of cytokines in SAP rats, alleviate the damage of intestinal mucosal barrier and enhance immune function.