Objective: SELDI-TOF-MS technology was used to contrastly analyse the changes of protein expression profiles of gastric cancer cells after TGF-β1 stimulation, and provide theoretical and experimental foundation for screening different significant proteins.
Methods: Serial subcultivation gastric cancer cells BGC823, MKN45, and SGC7901 cultured in vitro  were grouped into test and control groups.TGF-β1 was added to the test group, but  not to control group.Cell culture fluid was collected and centrifuged after cultured 24h, and crossing with WCX2 protein chip to detect protein differences. 
Results: When the test group was compared with control group, we found:(1) thirteen different proteins in BGC823 cells after TGF-β1 stimulaton, and their M/Z were M4294,M4932,M4945,M4972, M4991, M5015, 5036, M5060, M5153, M5180, M5197, M8577, and M8784, respectively; (2) eighteen different proteins in MKN45 cells after TGF-β1 stimulaton,and their M/Z were M4292,M4931,M4945，M4972，M4990, M5014, M5152, M5178, M7055, M8190, M8570, M8652, M8670, M8780, M9963, M10098, M10523, and M11653, respectively; (3) eight difference proteins in SGC7901 cells after TGF-β1 stimulation, and their M/Z were M4945，M4972，M4992，M5015, M5180, M7056, M8573, and M8604, respectively. By comparing three protein expression profiles of gastric cancer cells after TGF-β1 treatment,we found two significcant proteins with common differences,that had M/Z of M4945 and M4972, respectively.
Conclusions: The biological markers whose M/Z is M4945,M4972 with gastric cancer characteristic and associated with TGF-β1 have been screened,which can be as the basis for early prediction and clinical diagnosis research on metastasis and invasiveness of gastric cancer.