乳腺癌组织中 miR-21的表达及意义
作者:
作者单位:

作者简介:

吴诚义

通信作者:

基金项目:


Expression of miR-21 in breast carcinoma and its significance
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 音频文件
  • |
  • 视频文件
    摘要:

    目的:对乳腺癌组织及其相应癌旁组织中miR-21进行定量检测,分析其表达与临床病理特征的及意义。
    方法:采用茎环实时荧光逆转录聚合酶链反应(RT-PCR)检测60例乳腺癌及其对应癌旁组织中miR-21的表达量,分析其表达与肿瘤大小、TNM分期、组织学类型、淋巴结转移、雌激素受体(ER)、孕激素受体(PR)等临床病理特征的关系。
    结果:实时RT-PCR方法检测miR-21表达的敏感性和特异性良好。miR-21在乳腺癌组织中的表达明显高于癌旁组织(P<0.001);miR-21的高表达还与较高的TNM分期,淋巴结转移及PR的表达程度有关(各分组间P<0.01及< 0.05); miR-21的表达在“三阴性乳腺癌”(ER,PR,CerbB-2均为阴性)和“非三阴性乳腺癌”之间的表达存在统计学差异(P=0.029)。
    结论:miR-21在乳腺癌组织中高表达,其水平可能与乳腺癌的恶性程度有关。

    Abstract:

    Objective:To examine the level of expression of microRNA-21(miR-21) in breast cancer and its adjacent non-cancerous tissues, and analyze the miR-21 expression with the clinicopathological factors of breast cancer.
    Methods:Specimens of breast cancer and adjacent tissues were collected from 60 patients. Their miR-21 expression was detected by Stem-loop real-time RT-PCR, and levels of miR-21 expression in tumor size, TNM clinical stage, histology, lymph node involvement, and hormone receptor status were compared.
    Results:High sensitivity, specificity and precision of Stem-loop real-time RT-PCR allowed for direct analysis of expression of miR-21 in breast cancer tissues and adjacent tissues. The expression of miR-21 in breast cancer tissues was markedly higher than in adjancent tissue (P<0.001). Elevated miR-21 expression was also positively correlated with advanced TNM stage, lymph node metastasis, and low PR levels(P=0.007,0.001,and 0.012 respectively). Moreover, the levels of expression of miR-21 in triple-negative breast cancer(TNBC)were higher than those in non-TNBC types(P=0.029).
    Conclusions:High levels of expression of miR-21 are correlated with degree of malignancy of breast cancer.

    参考文献
    相似文献
    引证文献
引用本文

于超, 吴诚义.乳腺癌组织中 miR-21的表达及意义[J].中国普通外科杂志,2009,18(11):1126-1129.
DOI:10.7659/j. issn.1005-6947.2009.11.005

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2009-05-15
  • 最后修改日期:2009-08-26
  • 录用日期:
  • 在线发布日期: 2009-11-15