Objective:To study the antitumor effectivity of special cytotoxic T lymphocytes(CTLs) induced by B lymphocytes in mice.
Methods:B lymphocytes were collected, isolated and purfied. Cells were initially activated by CD40L and rmIL-4, then cocultivated with T lymphocytes. T lymphocyte proliferation was examined. Total RNA, which was extracted from Hepal-6(a hepatocelluar carcinoma cell line), were transfected into B lymphocytes, as experimental group; while transfected with RNA of mice liver cells,liposimes and 1640 were as control groups, The expression of antigen presenting cell(APC) markers (CD40,CD80 and CD86) and major histocomability complex(MHC) on B cell surface after transfection were deteced. CTL were obtained by stimulating T lymphocytes with transfected B lymphocytes. Hepal-6 was cell-targeted and examined as index of CTL killing activity. The IFN-r secretion of stimulated CTL was quantified.
Results: T cell proliferation in experimental group had a higher degree than that in RNA control group (P＜0.05), liposome control group and blank control group(P＜0.01). and the MHC and co-stimulating molecules expression of experimental group were significantly higher than other groups. Compared to control groups, the  killing activity and IFN-γ secretion of in experimental group was significantly increased (P＜0.05).
Conclusions:HCC RNA-transfected B lymphocyte has capability of inducing anti-tumor effects through CTL response, which may present a potential pathway for future HCC treatment.