Objective: To investigate the effct of PTTG gene on the growth and sensitivity to 5-FU of cholangiocarcinoma cell.
Methods: The pcDNA3.1-PTTGas that contained full-length antisense PTTG, and pcDNA3.1(+) was transfected into the cholangiocarcinoma cell line QBC939. We successfully established and identified cell sublines tQBC939(PTTG-), tQBC939(pcDNA3.1).  Cell growth curve was mapped out, cell proliferation rate was assessed by MTT assay and cell cycle percentage was counted by flow cytometry. After treatment with 5-FU, cell survival rate was assessed by MTT assay and IC50 was calculated. Flow cytometry and Hoechst staining were used to demonstrate discrepancy of cell apoptosis rates.
Results: Compared with other two groups, tQBC939 (PTTG-) grew more rapidly and had a higher proportion of cells in S phase, whereas less cells in G2/M phase（P＜0.05）. After treatment with 5-FU, IC50 of tQBC939(PTTG-) was significantly lower than that of other two groups, and increased rate of apoptosis was observed.
Conclusions: Anti-sense blocking PTTG enhances the growth of cholangiocarcinoma cell and sensitivity to 5-FU in vitro. The combination of anti-sense blocking PTTG with 5-FU can enhance the effect of chemotherapy in cholangiocarcinoma.