Objective: To detect the effect of exogenous IL-6 on the proliferation of cholangiocarcinoma cell line QBC939.
Methods: MTT was used to observe the proliferation of cell line QBC939 treated with exogenous IL-6. Following the annexin V/FITC and PI staining, FCM was used to detect the apoptosis of QBC939 treated with exogenous IL-6.
Results: The proliferation of cell line QBC939 was increased after treatment with IL-6 and was positively related with the concentration of IL-6（P＜0.01）. IL-6 could reduce the apoptosis rate of cell line QBC939 （P＜0.05）and upgrade the expression of  Bcl-2 mRNA.
Conclusions: IL-6, in a definite dose-range, could promote the proliferation of cell line QBC939, and this may be related with the upgrade of Bcl-2 mRNA.