Objective：To investigate the expression and significance of CD95 and JUK signal transducting system in liver cancer tissue.
Methods： Immunochemistry was employed to determine the expression of CD95, JNK1, JNK2 and c-Jun proteins in HCC tissues and adjacent normal liver tissues  in 58 cases of HCC.
Results：The staining of CD95 was located in cytoplasm, and JNK1, JNK2 and c-Jun were in nuclear. The expression of CD95,  JNK1, JNK2 and c-Jun in HCC was significantly lower than that in adjacent normal liver tissues (P<0.05).  In HCC, the negative expression of CD95 was 43(74.1%) and the positive expression was 15(25.9%). The negative expressions of JNK1, JNK2 and c-Jun were observed in 42（72.4%）, 39（67.2%）and 46（79.3 %） cases, respectively; with the positive expressions in 16（27.6%）, 19（32.8%）and 12（20.7%） cases, respectively. The CD95 expression level showed a positive correlation with the pathological grade of HCC（r=-0.516, P＜0.001); no significant relation was observed between CD95 with gender, age, number of nodules, venous infiltration or cirrhosis（P＞0.05）. The expression of JNK1, JNK2 and c-Jun respectively showed a negative correlation with the pathological grade of HCC（r=-0.364, P=0.006；r=-0.383, P=0.004；r=-0.508, P=0.002）. No significant relation was observed between CD95, JNK1, JNK2, c-Jun and gender, age, number of nodules, venous infiltration or cirrhosis（P＞0.05）.CD95 expression was positively correlated with expression JNK1（r=0.693, P＜0.001）, JNK2（r=0.357，P=0.007), and c-J un(r=0.670, P＜0.001）.
Conclusions：CD95 gene is significantly lower expressed in HCC tissue. CD95 protein may promote the initiation and development of HCC by modulating JNK signal transduction system.