Objective：To explore the effect of multidrug resistance (MDR) of human breast cancer Adriamycin resistant cell line MCF-7/ADM by interference gene DJ-1 expression.
Methods：DJ-1 shRNA plasmid was transfected into MCF-7/ADM cells by lipofectamine 2000; The mRNA and protein expression level of MDR1 was detected by RT-PCR and Western blot, respectively. The cellular sensitivity to chemotherapeutics was measured by MTT.
Results：Compared with blank and negative control groups  MDR1 mRNA and P-gp expression were reduced  after MCF-7/ADM cells interfered by DJ-1  sh′RNA,  (all P＜0.01). The chemosensitivity assays revealed that the transfected cells sensitivity to chemotherapeutics adriamycin was  increased 2.68 fold.
Conclusions：DJ-1 shRNA effectively reverses the multidrug resistance of human breast cancer Adriamycin resistant cell line MCF-7/ADM, which provides a novel approach for reversing breast cancer MDR.