Objective：To study the relevant differential expression proteins of GBC-SD cell line after treatment with thalidomide and the molecular mechanism.
Methods：Proteomic techniques and methods were used to separate the  differential expression proteins in GBC-SD cells after treated wiht that idomide (experiment group) and control group.
Results：The  differential expression proteins in experiment group and control group, whose spots variance was over 2-fold, were analysed  with PDQuest software,and found out 36 protein spots at difference level of expression（P＜0.05）, including 13 spots decreasing and 23 spots increasing  in experiment group. Then, 10 spots with more than 3-fold difference of  expression level were selected for anslysis, and 7 protein spots were identified.
Conclusions：Thalidomide has some effect on GBC-SD cells. Study on the function of differential protein can be as a basis for research on the mechanism of thalidomide treatment of gallbladder cancer.