Objective:To investigate the inhibitory effect of short hairpin RNAs (shRNAs) on tissue factor (TF) for affecting the invasion of gastric cancer. Methods: According to the principle of RNAi design， pSUPER/TF-siRNA expression vector was constructed， the recombinant plasmid shRNA was transfected into SGC-7901 cells (human gastric cancer cell line) with lipofectamine 2000. The mRNA and protein expression level of TF in SGC-7901 cells before and after transfection was determined by RT-PCR and Western blot respectively. The invasion ability in vitro was analyzed using Boyden chamber invasion assay. Results:The recombinant vector was constructed correctly， which was identified by double restriction enzymes digestion. The results of RT-PCR and Western blot showed that both mRNA and protein expression of TF were decreased significantly in SGC-7901 cells after transfected with pSUPER/TF-siRNA (P<0.05). Boyden chamber invasion assay showed that cell number of pSUPER/TF-siRNA/SGC-7901 invading through Matrigel filter was decreased significantly compared with that of pSUPER/SGC-7901 and SGC-7901 (P<0.05). Conclusions:TF is involved in the progression of gastric cancer， and it may be a new therapeutic target for gastric cancer.