Objective: To investigate the effect of the recombinant plasmid containing CD147-shRNA on the proliferative and migration ability of SMMC-7721 cells. Methods: The previously constructed and screened recombinant plasmid pBS/U6/CD147-shRNA3 was transfected into the SMMC-7721 cells. After that, the proliferation and migration ability of SMMC-7721 cells were detected by MTT and wound healing assay, respectively. Results: The MTT results showed that the proliferation of SMMC-7721 cells was significantly inhibited in a concentration dependent manner after transfection with pBS/U6/CD147-shRNA3 (0.3 and 1 μg), and the maximum percent inhibition was 32.2%. The scratch wound closure of the SMMC-7721 cells transfected with the control plasmid was almost complete after 24 hours and, in contrast, the scratch wound healing capability of the SMMC-7721 cells transfected with pBS/U6/CD147-shRNA3 was much weakened. Conclusion: CD147 gene silencing has inhibitory effect on the proliferation and migration of SMMC-7721 cells.