Objective: To determine the applicable scope of three types of surgical approach for laparoscopic cholecystectomy. Methods: The clinical data of 150 patients with benign gallbladder diseases undergoing laparoscopic cholecystectomy (LC) from January to December 2009 were retrospectively analyzed. Results: Of the 150 patients, laparoscopic cholecystectomy was performed via three-port approach in 46 cases, two-port approach in 54 cases, and transumbilical single-port approach in 50 cases. There were no differences among the three groups in the operation successful rate, duration of operation, intraoperative blood loss, and time for intestinal function recovery (all P>0.05). The transumbilical approach was better than the other two approaches in regards to postoperative pain and length of hospital stay (all P<0.05). No complications such as abdominal pain, jaundice, bile leakages, hemorrhage or incisional hernia occurred in any of the patients of the three groups during the follow-up period. Conclusion: Any of the three types of surgical approach can be used for the safe performance of laparoscopic cholecystectomy. However, the selection of the approach should be based on the patient’s individual condition and the principle of surgical safety.