Objective: To investigate the protective effect of salvianolate intravenous injection on the myocardial impairment secondary to acute necrotizing pancreatitis (ANP) in rats. Methods: Forty-two healthy male SD rats were equally randomized into the control group, ANP model group and salvianolate treatment group. The ANP model was induced by intraperitoneal injection of 20% L-Arginine solution and rats of the salvianolate treatment group were given salvianolate (100 mg/kg) injection through tail veil 10 min after ANP induction. All rats were killed 48 h after model establishment, and the serum amylase activity, contents of cardiac troponin I, and phospholipase A2 in myocardial tissues were detected. The pathological examination of the myocardial tissues was performed. Results: Compared with the control group, the activated serum amylase, and serum contents of cardiac troponin I and myocardial phospholipase A2 levels of both the ANP model group and salvianolate treatment group were significantly increased (all P<0.05), but these parameters of the salvianolate treatment group were all lower than those of the ANP model group (all P<0.05). No pathological change was noted in myocardial tissues of the control group, while the myocardial tissues of the ANP model group showed focal inflammatory infiltration with occasional myocyte rupture and hemorrhage, and the salvianolate treatment group exhibited slight inflammatory infiltration in interstitial tissue of myocardium. Conclusion: Salvianolate injection has protective effect on myocardial injury secondary to ANP.