Objective: To investigate the incidence of breast cancer among women aged 35–69 years in Guilin urban area. Methods: A total of 1 1167 Guilin area women, aged 35–69 years, underwent breast cancer screening by the modalities that included clinical breast examination, selective color ultrasound, selective molybdenum target mammography and pathological section examination. Results: Seven cases of breast cancer were detected from the whole group, so the detection rate was 62.68/100 000 (7/11 167). The detection rates in age group of 60–69, 50–54, 40–44, and 35–39 were 322.23/100 000, 57.87/100 000, 41.46/100 000, 76.80/100 000, respectively. No cancer was detected in the age group of 45–49 and 55–59. Conclusion: The incidence of breast cancer among women in Guilin is relatively high. The screening mode of this study can probably cause misdetection of no-lump breast cancer. In our opinion, the suitable screening mode for breast cancer in China still needs to be further explored and we suggest that the B-type ultrasound-based screening could be the mainstream for further development.