Objective: To evaluate the pathological changes of normal liver tissues surrounding the ablation zone after radiofrequency ablation (RFA) combined with hepatic blood inflow occlusion. Methods: VX2 liver tumor transplantation models were established in rabbits, and then they were assigned to undergo RFA alone (RFA group) or RFA plus hepatic blood inflow occlusion (RFA + hepatic occlusion group). RFA was performed using a cool-tip radiofrequency electrode (radiofrequency parameters setting: 30 W, 8 min), and hepatic blood inflow occlusion was done with the Pringle maneuver. The animals were sacrificed one week later and the pathological changes in liver tissues around the ablation zone were examined by using H&E staining. Results: Compared with RFA group, the surrounding liver tissues in RFA + hepatic occlusion group showed a larger number of inflammatory cells accumulating in the areas around central veins and Glisson’s sheaths, and other pathological changes such as liver cell swelling, expansion of portal vein and central vein, proliferation of bile duct epithelial cells were all more evident in RFA + hepatic occlusion group (all P<0.05). Conclusion: RFA plus hepatic blood inflow occlusion exerts more severe pathological damage in the liver tissues surrounding the ablation zone than RFA alone.