Objective: To assess the application value of the medical imaging software (SurgiCase 5.0) in liver surgery. Methods: The 128-slice spiral CT images of 10 patients with primary liver cancer admitted to our hospital over the past half year were collected. First, the data of slice-images were imported into SurgiCase 5.0 software for 3D remodeling. Then, these 3D models were used for planning of the surgical procedures, which were finally compared with the intraoperative findings. Results: The 10 3D models had better visualization, could be rotated and viewed from different angles, and clearly displayed the lesions as well as their spatial relationship with the intrahepatic vessels. The virtual models basically matched the actual intraoperative findings. The average volume of the resected liver of the 10 patients estimated by the software was (490.1±185.9) mL and the average volume actually resected was (464.4±169.5) mL with error rate of 7.1%, and there was a significant positive correlation between them (r=0.993, P<0.01). Conclusion: SurgiCase 5.0 can provide a reference for complex hepatic resections, and help in choosing the correct surgical procedure and decrease surgical risk.