Objective: To assess the standardization of lymphadenectomy in hand-assisted laparoscopic radical gastrectomy for stomach cancer using nanocarbon particles as lymph node (LN) tracer. Methods: The clinical data of 40 patients with gastric cancer undergoing hand-assisted laparoscopic surgery for radical gastrectomy within a period of 8 months were retrospectively analyzed. Of the patients, 15 cases who received intraoperative nanocarbon suspension injection before LN clearance were designated as observation group, and another 25 cases served as control group. Results: Of the 15 patients in observation group, the LNs along the lesser curvature of the stomach were labeled in 12 cases (80.00%), No.8a or No.6 LNs staining were found in 8 cases each (53.33%), and the LNs staining were found in 3 cases each (20.00%) in the splenic hilum or greater curvature of the stomach. All the main surgical indexes that included the incision length [(6.90±0.28) cm vs. (6.82±0.38) cm], intraoperative blood loss [(286±168) mL vs. (235±98) mL], operative time [(182±31) min vs. (176±21) min], number of lymph nodes detected (16.8±6.71 vs. 14.9±3.90), and length of postoperative hospital stay [(10.1±3.8) d vs. (11.6±6.5) d], between the observation group and control group showed no statistical differences (all P>0.05). Conclusion: The application of LN tracer has certain adjunctive effects for improving the LN dissection during surgery. The use of tracer in hand-assisted laparoscopic radical gastrectomy can better reflect the standardization of LN dissection.