Objective: To investigate the expressions of uPA and vimentin protein in hepatocellular carcinoma (HCC) and their clinicopathologic significance. Methods: The expressions of uPA and vimentin protein in HCC along with their paired adjacent tissues (88 cases) and normal liver tissues (8 cases) were determined by immumohistochemical staining. The relations of the expressions of the both proteins with the clinicopathologic characteristics of HCC patients, and the correlation between themselves were analyzed. Results: The positive expression rates of uPA and vimentin protein in HCC tissues were 63.6% and 72.7% respectively, and both were significantly higher than those in their adjacent tissues (27.3% and 15.9%) and normal liver tissue (12.5% and 12.5%) (all P<0.0125). The expressions of uPA and vimentin protein in HCC were associated with the presence of portal vein tumor thrombus and liver capsule invasion (all P<0.05), and there was a positive correlation between the expressions of uPA and vimentin protein in HCC tissues (r=0.227, P=0.034). Conclusion: The expressions of uPA and vimentin protein in HCC are positively correlated and both positive expressions are associated with the unfavorable clinical characteristics of the patients.