Objective: To investigate the management of biliary re-stricture after an initial repair of iatrogenic bile duct injury. Methods: The clinical data of 22 patients with biliary re-stricture after repair of bile duct injury in other hospital admitted from January 2001 to December 2011 were retrospectively analyzed. The bile duct injury in all the 22 cases was caused by cholecystectomy that included 8 cases of laparoscopic cholecystectomy and 14 cases of open cholecystectomy. Results: Reoperations were performed in all the 22 patients, of whom 21 cases underwent Roux-en-Y hepaticojejunostomy (one case underwent quadrate lobectomy due to high injury site of the bile duct, and then anastomosis of the left and right hepatic ducts to the jejunum), and one case received U-tube external biliary drainage. All patients were followed up for 1 year to 11 years. Except for one patient who died of liver failure caused by biliary infection, all the remaining patients showed relatively positive short-term outcomes. Conclusion: Bile duct injury should be treated as early as possible, and attention to detail during operation and proper selection of repair method is important. Roux-en-Y hepaticojejunostomy is a reliable procedure for biliary re-stricture after repair of the bile duct injury.