Objective: To make a preliminary evaluation for the short-term efficacy of hand-assisted laparoscopic radical gastrectomy. Methods: The clinical and follow-up records of 100 stomach cancer patients undergoing hand-assisted laparoscopic radical gastrectomy from December 2010 to July 2012 were retrospectively analyzed. Results: Hand-assisted laparoscopic radical gastrectomy was performed in 102 patients, of whom 100 cases were completed successfully and two cases (1.96%) were converted to open procedure. Among the 100 laparoscopic patients, gastrectomy was performed in 53 cases, distal gastrectomy in 39 cases, and proximal gastrectomy in 8 cases; 4 cases underwent combined cholecystectomy, 2 cases underwent combined partial resection of the left lateral lobe, and one case underwent combined splenectomy plus resection of pancreatic body and tail. The average length of incision was (6.81±0.29) cm, blood loss was (244.10±117.02) mL, operative time was (172.28±24.02) min, number of lymph nodes obtained for pathological examination was 17.10±5.70, and length of postoperative hospital stay was (10.20±3.84) d, respectively. No perioperative death occurred and the incidence of postoperative complications was 9% (9/100). The postoperative follow–up period ranged from one month to 12 months, and 6 cases (6%) were lost to follow-up. Simultaneous liver metastases were found in 3 cases, heterochronic lymph node metastasis occurred in one case, and local recurrence developed in one case, respectively. Conclusion: Hand-assisted laparoscopic D2 radical surgery for gastric cancer is safe and reliable with favorable short-term outcome, and it may not increase the chance of intra-abdominal metastases.