Objective: To investigate the clinical efficacy of single loop jejunal interposition following laparoscopic radical gastrectomy for advanced gastric cancer. Methods: The data of 21 patients with advanced gastric cancer treated from January 2011 to June 2013 were reviewed. All patients underwent total gastrectomy followed by single loop jejunal interposition for digestive tract reconstruction, and the clinical variables that included postoperative complications, postoperative nutritional status and gastrointestinal symptoms were analyzed. Results: Operations were successfully completed in the 21 patients, and all of them were discharged from hospital after recovery. No postoperative complications such as anastomotic fistula, dumping syndrome or reflux esophagitis occurred. Six months after surgery, gastroscopy and upper gastrointestinal tract contrast examination showed that the anastomotic stomas of the patients were smooth and patent, and the patients had satisfactory nutritional status and favorable quality of life. Conclusion: Single loop jejunal interposition after laparoscopic radical gastromy for advanced gastric cancer is an ideal reconstruction with respect to improving the postoperative gastrointestinal symptoms and quality of life.