Objective: To investigate the feasibility and safety of Glissonean pedicle transection method for laparoscopic hepatectomy. Methods: The clinical data of 18 patients undergoing laparoscopic liver resection with Glissonean pedicle transection method between January 2011 and June 2013 were retrospectively analyzed. Results: All of the 18 patients successfully underwent laparoscopic left hemihepatectomy or left lateral lobectomy using Glissonean pedicle transection method, and without any open conversion. The operative time was 42-300 min with an average of (215.6±56.6) min, the intraoperative blood loss was 50-200 mL with an average of (118.6±50.5) mL and without a need for blood transfusion, the length of hospital stay was 8-16 d with an average of (11.4±3.1) d, and there was no occurrence or complications. The patients were followed-up for 3-24 months, all of them were alive and had a satisfactory quality of life, and no tumor recurrence occurred in the 6 cases with hepatocellular carcinoma. Conclusion: Glissonean pedicle transection method can effectively control bleeding, and the use of this method in laparoscopic anatomic left hemihepatectomy or left lateral lobectomy is safe and feasible.