Objective: To systematically assess the association between the gene polymorphism of glutathione S-transferases T1 (GSTT1) and gastric cancer susceptibility in Chinese population. Methods: The case-control studies on relationship between GSTT1 gene polymorphism and gastric cancer susceptibility in Chinese population were collected by searching several national and international databases. Meta-analysis was performed according to the GSTT1 genotype distributions in case group and control group. Results: Seventeen case-control studies were finally selected, with a total of 2 468 gastric cancer patients in case group and a total of 4 745 healthy subjects in control group. Results of the Meta-analysis indicated that the risk of gastric cancer was increased in subjects with GSTT1 null genotype compared with those with GSTT1 present genotype, and the pooled OR in fixed-effect model and random-effects model was 0.84 (95% CI=0.76–0.93) and 0.83 (95% CI=0.71–0.96), respectively. Results of subgroup analysis according to sample size showed that the pooled OR was 0.87 (95% CI=0.77–0.98) for studies with sample size larger than 100 and was 0.77 (95% CI=0.63–0.94) for studies with sample sized equal to or less than 100, respectively. Conclusion: GSTT1 gene polymorphism is closely associated with gastric cancer susceptibility in Chinese population, and those with GSTT1 null genotype face an increased risk of gastric cancer.