Objective: To evaluate the feasibility of using indocyanine green (ICG) fluorescence technique in sentinel lymph node biopsy (SLNB) for breast cancer. Methods: Sixty-eight breast cancer patients admitted between November 2010 and February 2012 were selected. Of the patients, 36 cases underwent SLNB with ICG fluorescence navigation (ICG group), while 32 cases underwent SLNB using methylene blue as a tracer (dye group). All patients received level I and II axillary dissection after the SLNB. Results: The baseline clinical data between the two groups had no statistical difference (all P>0.05), and were comparable. The detection rate of sentinel lymph nodes (SLNs) was 97.2% (35/36) in ICG group and 81.3% (26/32) in dye group, and the former was significantly higher than the latter (P<0.05). There was no significant difference in false-negative rate and mean number of excised SLNs between the two groups (both P>0.05). Conclusion: The detection rate of using ICG fluorescence navigation SLNB for breast cancer is higher than that of dye tracer, and it can be used as an alternative option for radioactive or dye tracer.