Objective: To investigate the expression and clinical significance of hypoxia inducible factors 1α (HIF-1α) and glucose transporter 1 (Glut-1) in basal-like breast carcinoma (BLBC). Methods: The protein expressions of HIF-1α and Glut-1 in 95 specimens of BLBC and their adjacent breast tissue, and 708 samples of non-BLBC tissue were detected by immunohistochemical staining, and their relations with the clinicopathologic factors of BLBC were analyzed. Results: The positive expression rate of either HIF-1α or Glut-1 protein in BLBC tissue was significantly higher than that in adjacent breast tissue and non-BLBC tissue (all P<0.05), and both HIF-1α and Glut-1 protein expressions were associated with lymph node metastasis and clinical stage of BLBC (all P<0.05); there was a significantly positive correlation between HIF-1α and Glut-1 expression in BLBC tissue (r=0.368, P<0.05). Conclusion: HIF-1α and Glut-1 synchronously overexpress in BLBC tissue, which may co-participate in the occurrence and development of BLBC, and can be potentially used as a prognostic index for BLBC patients.