Objective: To evaluate the clinic value of using the technique of “laparoscopic meticulous dissection of Calot’s triangle via cystic artery approach” for preventing bile duct injury during laparoscopic cholecystectomy (LC). Methods: The clinical data of 2 200 patients undergoing LC with this technique from June 2010 to June 2014 were analyzed retrospectively. The essential feature of the technique was namely “cystic artery first”, and full exposure of the relationship of the three biliary ducts after meticulously dissecting out the contents of Calot’s triangle. Results: LC procedure was successfully completed in 2 152 patients, without bile duct injury occurring in any of them, and the other 48 patients (2.18%) were converted to open surgery due to Mirizzi’s syndrome, cholecystoduodenal fistula or gallbladder cancer The operative time was 20 to 55 min, with an average of 35.5 minutes, the intraoperative blood loss was 5 to 50 mL, with an average of 15.5 mL, and the length of postoperative hospital stay was 3 to 5 d, with an average of 3.5 d, respectively. Follow-up performed for 3 to 12 months, and no complications such as biliary stricture, bile leakage or intraperitoneal abscess occurred. Conclusion: Using the technique of “laparoscopic meticulous dissection of Calot’s triangle via cystic artery approach” can effectively prevent bile duct injury during LC, and it can be recommended as a standard procedure.