Objective: To assess the clinical feasibility of transumbilical single port laparoscopic cholecystectomy. Methods: The clinical data of 85 patients with gallstones or gallbladder polyps undergoing laparoscopic cholecystectomy between August 2012 and October 2013 were retrospectively analyzed. Of the patients, 41 cases underwent transumbilical single port laparoscopic cholecystectomy (single-port group), and 44 cases underwent traditional three-port laparoscopic cholecystectomy (three-port group). The relevant clinical variables between the two groups were compared. Results: All laparoscopic procedures were successfully conducted, without any open conversion. The operative time in single-port group was significantly longer than that in three-port group [(31.73±4.22) min vs. (15.43± 1.81) min, P=0.000], and intraoperative blood loss, length of postoperative hospital stay, hospitalization costs and incidence of wound infection showed no statistical difference between the two groups (all P>0.05), while the ratio of patients receiving analgesics in single-port group was significantly reduced compared with three-port group (7.31% vs. 34.09%, P=0.003). No postoperative biliary fistula or other serious complications occurred in any of the two groups. Conclusion: Single-port laparoscopic cholecystectomy has the same therapeutic effect as traditional three-port laparoscopic cholecystectomy, but it has evident superiority in minimal invasiveness.