Objective: To investigate the clinical value of penetrating-suture pancreaticojejunostomy. Methods: The clinical data of 83 patients undergoing pancreaticoduodenectomy (PD) from May 2006 to July 2014 were retrospectively analyzed. All patients received penetrating-suture pancreaticojejunostomy for pancreatic-enteric anastomosis during surgery, which was characterized by anastomosis of the cutting surface (not the cutting margin) of the pancreas to the jejunal wall, and anastomosis of the pancreatic duct to the intestinal mucosa. Results: Of 83 patients, 32 cases were carcinoma of the pancreatic head, 42 cases were periampullary carcinoma and 9 cases had other conditions; 81 cases underwent radical pancreaticoduodenal resection and two cases had non-radical resection. The operative time was 220 to 350 min, with an average of 290 min, and the time for pancreatic-enteric anastomosis was 6 to 22 min, with an average of 8 min. According to ISGPF criteria, clinically significant postoperative pancreatic fistula occurred in 8 cases, and all were grade B simple pancreatic fistulas. Bile leakage occurred in 2 cases and delayed gastric emptying occurred in 6 cases. No reoperation was needed and no anastomotic bleeding or surgical death occurred. Conclusion: The technique of penetrating-suture pancreaticojejunostomy can effectively prevent postoperative anastomotic failure at the pancreatic-enteric anastomosis and anastomotic bleeding.