Objective: To determine the risk factors for calcification in breast cancer. Methods: The clinicopathologic and imaging data of 196 patients with pathologically confirmed breast cancer between 2012 and 2014 by pathological diagnosis were retrospectively analyzed. The relations of clinicopathologic factors with breast calcification were determined by univariate and multivariate Logistic regression analysis. Results: In 196 patients, 140 cases (71%) had breast calcification and 56 cases (29%) had no breast calcification. Univariate analysis showed that lymph node metastasis, TNM stage, pathological type, and HER-2 status were associated with calcification (all P<0.05). Multivariate Logistic regression analysis revealed that pathological type (OR=0.299, 95% CI=0.134–0.665), tumor diameter (OR=2.436, 95% CI=1.108–5.357), and HER-2 expression (OR=2.827, 95% CI=1.363–5.864) were inferential factors for calcification (all P<0.05), and the calcification risk of invasive ductal carcinoma (IDC) was 0.299-fold that of ductal carcinoma in situ (DCIS), tumor ≥2 cm was 2.436-fold that of tumor <2 cm, positive HER-2 expression was 2.827-fold that of negative HER-2 expression. The constituent ratio of simple calcification was increased in patients with tumor size ≤2 cm, DCIS or HER-2 positive expression, and the constituent ratio of calcification with mass was higher than other calcification types in patients with IDC or tumor size >2 cm (all P<0.05). Conclusion: DCIS, HER-2 expression and tumor diameter are main risk factors for calcification in breast cancer.