Objective: To investigate the expressions of HSP27 and claudin-10 in colorectal carcinoma (CRC) and the clinical significance. Methods: The expressions of HSP27 and claudin-10 in 50 specimens of CRC tissue, 25 specimens of colorectal adenoma (CRA) tissue, and 50 specimens of normal colorectal mucosal tissue were determined by immunohistochemical staining. The relationship between HSP27 and claudin-10 expressions in CRC, and the relations of HSP27 and claudin-10 expressions with clinicopathological factors and prognosis of the CRC patients were analyzed. Results: In CRC, CRA and normal colorectal mucosal tissue, the positive expression rate of HSP27 was 54%, 20% and 16% respectively, with statistical significance (P<0.001), and the positive expression rate of claudin-10 was 72%, 56% and 54% respectively, with no statistical difference (P>0.05). There was a positive correlation between HSP27 and claudin-10 expressions in CRC tissue (r=0.318, P=0.024). Univariate analysis showed that positive HSP27 expression was associated with lymph node metastasis (P<0.05), and the positive claudin-10 expression was related to tumor diameter, invasion depth and lymph node metastasis (all P<0.05). Survival analysis showed that the mean survival time in CRC patients with positive HSP27 or claudin-10 expression was significantly shorter than in those with its negative expression (both P<0.05). Multivariate analysis showed that the HSP27 and claudin-10 expressions together with lymph node metastasis were the independent risk factors for the prognosis of the CRC patients. Conclusion: HSP27 expression is up-regulated in CRC tissue, which may probably be synergistic with claudin-10 to exert important effect on lymph node metastasis in CRC.