Objective: To investigate the golgi phosphoprotein 3 (GOLPH3) expression in colorectal cancer tissues and its relation with prognosis of colorectal cancer. Methods: The GOLPH3 along with the c-Myc and P27 expressions in 123 specimens of colorectal cancer were determined by immunohistochemical staining, and the relations of GOLPH3 expression with the clinicopathologic features, the c-Myc and P27 expressions and the prognosis of the patients were analyzed. Results: The positive expression rate of GOLPH3 in these colorectal cancer tissues was 60.2% (74/123). The positive GOLPH3 expression was significantly related to tumor invasion depth, TMN stage, lymph metastasis and distant metastasis as well as c-Myc expression (all P<0.05), but irrelevant to sex, age, tumor size, differentiation, CEA level and P27 expression (all P>0.05). The GOLPH3 expression was positively correlated with c-Myc expression in colorectal tissue (r=0.263, P<0.05). The 5-year overall survival rate was 56.9% for the entire group of patients, was 48.6% for patients with positive GOLPH3 expression and 69.4% for those with negative GOLPH3 expression. Univariate survival analysis showed that the survival rate in patients with positive GOLPH3 expression was significantly lower than that in patients with negative GOLPH3 expression (P<0.05). Conclusion: GOLPH3 expression may have a synergistic action with c-Myc in colorectal cancer and those with positive GOLPH3 expression may face an unfavorable prognosis.