Objective: To compare the incidence of perioperative complications and long-term efficacy between laparoscopic and open surgery in treatment of Crohn’s disease (CD). Methods: The relevant non-randomized controlled trials (nRCTs) were collected by searching several Chinese and English online databases. The studies were screened, data were extracted, and the quality was evaluated according to the Cochrane Reviewers Handbook 5.0, and then Meta-analysis was made by using RevMan 5.2 software. Results: Twenty-three nRCTs were finally included involving 2 101 patients, of whom, 1 006 cases underwent laparoscopic surgery (laparoscopic surgery group) and 1 095 underwent open surgery (open surgery group). Results of Meta-analysis showed that the incidence of perioperative complications was significantly decreased in laparoscopic surgery group compared with open surgery group (RR=0.74, 95% CI=0.6-0.91, P=0.004); in long-term follow-up, the incidence of postoperative incisional hernia in laparoscopic surgery group was significantly lower than that in open surgery group (RR=0.21, 95% CI=0.07-0.68, P=0.009), while the disease recurrence rate and incidence of bowel obstruction had no statistical difference between the two groups (both P>0.05). Conclusion: For CD, laparoscopic surgery is superior to open surgery in reducing perioperative complications and postoperative incisional hernia.