Objective: To establish a stable 30% reduced-size liver transplantation model in rats. Methods: Using male SD rats of similar weight, the reduced-size grafts were obtained by resecting the hepatic left and medial lobe in vivo, with preservation of the caudate, right and triangle lobe of the liver, and liver implantation was performed by two-cuff technique, respectively. The wet weight ratio of graft to the entire liver was determined, and the 2- and 7-d post-implantation survival rates were observed. Results: A total of 80 reduced-size liver transplantation rat models were created. The wet weight of graft and entire liver was (4.02±0.64) g and (11.84±1.36) g respectively, and the wet weight ratio graft to entire liver was 33.81%, with the 95% confident interval of 33.22% to 34.40%. The 2- and 7-d post-implantation survival rate of the recipient was 84% and 66% respectively, and the main causes of death included abdominal hemorrhage, bile leakage, abdominal infection, chronic liver graft dysfunction, and pulmonary infection. Conclusion: The method of resecting left and middle lobe can maximally reduce the non-functional remnant liver, with weight ratio of remnant liver to entire liver approximating to 30%, by which the stable reduced-size liver transplantation model can be established.