Objective: To investigate the affecting factors for lymphovascular tumor cell invasion and its relation with prognosis in patients with stage II colon cancer. Methods: The clinicopathologic data of 152 patients with stage II colon cancer who underwent radical surgery in the Fourth Affiliated Hospital of China Medical University from January 2007 to August 2010 were collected. The relations of lymphovascular tumor cell invasion with clinicopathologic variables and the prognostic factors of the patients were analyzed. Results: The 5-year overall survival rate in the whole group of patients was 73.7%, which in patients with IIa, IIb and IIc disease was 79.6%, 73.3% and 65.8%, respectively. Statistical analysis showed that lymphovascular tumor cell invasion was significantly associated with tumor site, degree of differentiation and T stage of colon cancer (all P<0.05). Univariate analysis indicated that degree of differentiation, positive surgical margin, lymphovascular tumor cell invasion, number of detected lymph nodes and T stage were prognostic factors for patients with stage II colon cancer (all P<0.05); multivariate analysis identified that T stage, positive surgical margin and lymphovascular tumor cell invasion were independent prognostic factors for patients with in stage II colon cancer (all P<0.05). The 5-year survival rate in patients with positive lymphovascular invasion was significantly lower than in those without lymphovascular tumor cell invasion (52.7% vs. 85.6%, P<0.05). In addition, the 5-year survival rate in cases with positive lymphovascular tumor cell invasion undergoing chemotherapy was significantly higher than in those without chemotherapy (60.7% vs. 44.4%, P<0.05). Conclusion: In patients with stage II colon cancer, lymphovascular tumor cell invasion is related to tumor site, degree of differentiation and T stage, which is an independent risk factor for their prognosis. Adjuvant chemotherapy is recommended for stage II colon cancer patients with positive lymphovascular tumor cell invasion.