Objective: To investigate the feasibility, safety and efficacy of total laparoscopic choledochal cyst excision with Roux-en-Y hepatoenterostomy for congenital choledochal cysts in adult patients. Methods: The clinical data of 12 patients who underwent laparoscopic choledochal cysts excision from May 2014 to May 2016 in the First Affiliated Hospital of Kunming Medical University were analyzed retrospectively. Results: Of the patients, total laparoscopic choledochal cyst excision with Roux-en-Y hepatoenterostomy was successfully performed in 11 cases, and one case had extra-abdominal manual enteroenterostomy to reduce surgical cost. The operative time was 240 to 310 min, with an average of 280 min, the intraoperative blood loss was 20 to 150 mL, with an average of 60 mL and the length of postoperative hospital stay was 5 to 7 d, with an average of 6.5 d. Postoperative follow-up was conducted for 2 to 12 months, and no surgical complications or death occurred. Conclusion: Total laparoscopic choledochal cyst excision with Roux-en-Y hepatoenterostomy is safe and feasible in treatment of adult congenital choledochal cysts, and has obvious minimally invasive advantage. So, it is recommended to be used.