Objective: To investigate the application efficacy of vascular occlusion of the hepatic lobe in anatomical right hepatectomy. Methods: The clinical data of 12 patients were analyzed retrospectively. Of the patients, 8 cases were primary liver cancer and 4 cases were hepatolithiasis. In all the patients, the lesions were confined to the right lobe of the liver or one segment of the right liver. All patients underwent anatomical right hepatectomy with vascular occlusion of the hepatic lobe. Results: Operations in the 12 patients were all successfully completed, which included segment V resection in one case, segment VII resection in 2 cases, segment VIII resection in 3 cases, right posterior lobectomy in 4 cases and right anterior lobectomy in 2 cases. The average operative time was (263.33±27.99) min, and the average intraoperative blood loss was (397.50±85.29) mL. After operation, hydrothorax and bile leakage occurred in one case each, which were resolved by conservative treatment. All patients recovered and were discharged from the hospital. Conclusion: Using vascular occlusion of the hepatic lobe in anatomical right hepatectomy can effectively control the intraoperative bleeding and reduce the liver function damage, so it is safe and feasible.