Objective: To investigate the expression of long non-coding RNA BCAR4 (LncRNA BCAR4) in pancreatic carcinoma cells and its effects on proliferation and apoptosis of pancreatic carcinoma cells. Methods: The expressions of LncRNA BCAR4 in different pancreatic carcinoma cell lines (AsPC-1, HPAC, BxPC-3 and Panc-1) and normal pancreatic duct epithelial cells HPDE6-C7 were determined by qRT-PCR method. The pancreatic carcinoma AsPC-1 cells were transfected with BCAR4 siRNA or crambled sequences, with untransfected AsPC-1 cells as blank control, and then the cell proliferation and apoptosis were measured by CCK-8 assay and flow cytometry, and the protein expression levels of p-mTOR and p-P70S6K as well as phosphorylated mTOR (p-mTOR) and phosphorylated P70S6K (p-P70S6K) were detected by Western blot analysis. Results: The relative expression levels of LncRNA BCAR4 in all studied pancreatic carcinoma cell lines were significantly higher than that in normal pancreatic duct epithelial HPDE6-C7 cells (all P<0.05). After BCAR4 siRNA transfection, AsPC-1 cells exhibited decreased proliferation, increased apoptosis rate and down-regulated p-mTOR and p-P70S6K expressions (all P<0.05). Conclusion: LncRNA BCAR4 expression is increased in pancreatic carcinoma cells. High LncRNA BCAR4 expression can promote proliferation and inhibit apoptosis of the pancreatic carcinoma cells, and the mechanism may be associated with its up-regulating phosphorylation level of mTOR/P70S6K pathway.