Objective: To investigate the application efficacy of laparoscopic modified intersphincteric resection (ISR) combined with transanal pull-through in anus-preserving operation for low rectal cancer. Methods: The clinical data of 11 patients with low rectal cancer undergoing laparoscopic modified ISR combined with transanal pull-through from April 2016 to June 2017 were retrospectively analyzed. Results: Operation was successfully performed in all the 11 patients. The average operative time was 215 (18–320) min, the average blood loss was 52 (30–250) mL, and the average length of postoperative hospital stay was 11.6 (9–15) d. No anastomotic leakage, presacral infection, anastomotic stricture or postoperative hemorrhage occurred. The number of obtained lymph nodes in single specimen ranged from 12 to 26 with an average number of 15. The 11 patients were followed up for 4 to 16 months, with an average period of 10 months. No surgery-associated complications were found, and no local recurrence or metastasis occurred. The anal functions of the patients were satisfactorily recovered after 6 months. Conclusion: Laparoscopic modified ISR combined with transanal pull-through is an ideal procedure for low rectal cancer with anus preservation. It offers satisfactory anal function and effect with no requirement of preventive colostomy, and can completely avoid the occurrence of anastomotic leakage, with the advantages of small trauma, quick recovery and low costs.