The outbreak of novel coronavirus (SARS-CoV-2)-infected pneumonia (NCP, WHO named as COVID-19) with a concentrated eruption in Wuhan, China has posed a serious threat to the health of people, which has so far spread to 25 countries and regions. As of 13 February 2020, the cumulative number of confirmed cases was 63 946 and the cumulative number of suspected cases was 10 109, with a fatality rate of 2.06%. The main routes of transmission of SARS-CoV-2 are currently considered to be by droplets and close contacts, while the possibility of aerosol or fecal oral transmission still cannot be ruled out. The latest study indicated that the NCP has a very high transmission rate (R0=3.77). According to regulations issued by the National Health Commission, the surgical operations should be confined to emergency or urgent cases during the epidemic period. At present time, there are no relevant guidelines or recommendations as well as available experiences in China and abroad for when the operations are performed and how the perioperative prevention and protection are carried out in patients with suspected or confirmed NCP. According to incomplete statistics, there were a total of 50 perioperative patients with NCP admitted in the Zhongnan Hospital of Wuhan University and Renmin Hospital of Wuhan University during the epidemic period. Of the patients, 2 cases with NCP diagnosed after operation infected 16 people who were medical staff or relevant personnel, and 48 cases with suspected or confirmed NCP before operation did not infect any of the medical staff or relevant individuals after specific prevention and protection measures were implemented. Here, the authors based on the quarantine measures framed during the NCP epidemic and the prevention and protection experiences of Xiangya Hospital, and combined with the data of cases from the above two hospitals in Wuhan, discuss how to carry out a standard operation and make effective prevention and protection measures during perioperative period under the epidemic situation of NCP.