Background and Aims: Intraoperative bleeding is the main cause of conversion to laparotomy in anatomical laparoscopic hepatolobectomy (ALH), and effective control of blood inflow to the liver is the key step to achieve successful implementation of ALH. Traditionally, the hepatic inflow occlusion for ALH follows the conception of “Glissonean pedicle-first”, namely, isolation and division of the Glissonean pedicle (specifically, it can be divided into "intra-Glissonian approach" and "extra-Glissonian approach") are performed prior to liver parenchymal transection. However, this method is complicated and time-consuming. In the long-term clinical work, the authors have explored a new technique for hepatic inflow occlusion and named it as the "liver parenchymal dissection-first" (LPDF) method. This method just adjusts the order of operative procedures without expanding the scope of the operation, and it can simplify the hepatic inflow occlusion, and thereby facilitate the promotion and application of ALH. This paper was aimed to introduce a case of laparoscopic anatomical mesohepatectomy with LPDF method and preliminarily summarize the technical characteristics of LPDF. 
Methods: The clinical data of a patient with hepatocellular carcinoma (HCC) undergoing anatomical laparoscopic mesohepatectomy using LPDF technique in October 2019 were retrospectively analyzed. The HCC lesions in the patient involved the segments 8, 5 and 4b. During the operation, the perihepatic ligaments were dissociated, then the first porta hepatis was blocked, the liver parenchyma was fully dissected along the right side of the falciform ligament and toward the first and second porta hepatis (the left resection line) using ultrasonic scalpel until the exposure of a proper length of the intrahepatic right anterior Glissonean pedicle, which was then ligated and transected. As a result, the demarcation line of the right anterior lobe was appeared (the right resection line), and the right parenchymal resection was performed. Finally, the middle hepatic lobe and the lesions were completely resected after the intersection of the two cutting planes.
Results: The operative time was 260 min, the amount of intraoperative blood loss was 300 mL, and no blood transfusion was needed. The bowel function was recovered on postoperative day (POD) 3, and the patient was discharged from the hospital on POD 6. There were no complications such as massive intraperitoneal hemorrhage and bile leakage occurred. One month later, a 117 mm×87 mm fluid collection in the operation area was found by color doppler ultrasound, but no fever or abdominal pain was noted, and the number of white blood cells and level of total bilirubin were within the normal ranges. The fluid was gradually absorbed without treatment. 
Conclusion: LPDF is safe and feasible. It can facilitate the extra-Glissonian inflow occlusion for laparoscopic anatomical mesohepatectomy, and its application value in ALH is worthy of further exploration.