Abstract:Objective To investigate the expression of oncogenes and tumor suppressor genes in hepatitis B virus (HBV) related hepatocellular carcinoma (HCC). Methods mRNA was extracted from cancerous and paracancerous tissues of 22 patients with HBVrelated HCC and synthesized into cDNA. The cDNA labeled with 33pdATP was hybridized for cDNA microarray each consisting of 3170 genes or expressed sequence tags (EST). The differential expression genes were searched in gene data base and verified using RTPCR. Results The differential expression of 1369 genes or EST was identified including 121 genes or EST altered 2 times or more in cancerous tissues compared with paracancerous tissues. Compared with paracancerous tissues, 88 showed overexpression and 33 genes were downregulated. The positive expression of transmembrane 4 superfamily member 1 (TM4SF1) in cancerous tissues was 86.4% and could not be detected in paracancerous tissues （P＜0.001）. The positive expression of suppression of tumorigenicity 14 (ST14) in paracancerous tissues of HBVrelated HCC patients was 72.8% and could not be detected in cancerous tissues （P＜0.01）. Conclusions The positive expression of TM4SF1 in cancerous tissues，and ST14 in paracancerous tissues of HCC were related to the development of HBVrelated HCC.