Abstract:Objective To evaluate the role of hepatocyte apoptosis in ischemia/reperfusion injury of grafted liver and the effects of endothelin1 (ET1) monoclonol antibody on hepatocyte apoptosis.
Methods Othotopic liver transplantation rats were divided into two groups: with and without ET1 antibody. The concentrations of ET1 of plasma and liver tissue were measured. The parameters of liver fuction were determined. The concentration of malondialdehyde (MDA) was measured and the number of apoptotic hepatocytes in grafts liver was determined.
Results The levels of ET1, ALT in serum, and ET1, MDA and apoptotic cells in the grafted liver after ischemia/reperfusion were significantly increased compared with normal values. In the ET1 antibody group, the levels of ET1, ALT, MDA and apoptotic cells were significantly decreased. 〖WTHZ〗Conclusions ET1 monoclonal antibody can attenuate ischemia/reperfusion injury by decreasing lipid peroxide reaction, and decreasing apoptosis of hepatocytes and thus protect the liver graft.