Abstract:Objective
To study arsenic trioxide induces apoptosis of human cholangiocarcinoma cell line QBC939.
Methods MTT was applied to detect the inhibitory effect of arsenic trioxide on cholangiocarcinoma cell line QBC939. Morphological changes were observed by light microscopy and fluorescence microscopy. Rhodamine123 dyeing and analysis of DNA content and cell cycle were examined by flow cytometry. Results Cell growth was significantly inhibited by different concentration(116μmol/L) of arsenic trioxide. The inhibition ratio was dependent on arsenic concentration and time. The typical morphological character of apoptosis was observed, apoptotic peak of hypodiploid was detected and fluorescence intensity of Rhodamine123 decreased when the cells were cultured with 4μmol/L As2O3.
Conclusions As2O3 can induce apoptosis of cell line QBC939. The mechanism may be related to the depolarization of mitochondrial membrane potential.