Abstract:Objective To investigate a new microcapsular system for culture of primary generation hepatocytes in vitro.
Methods A novel microcapsular system was used for in vitro culture of primary generation rat hepatocytes.The various characteristics of the microcapsular system were examined. The survival rate of hepatocytes and the functions of urea and albumin synthesis were observed.
Results The microcapsule had good permeability,a certain degree of mechanical stability and favorable immunological protective ability.Hepatocytes within the microcapsule maintained a high degree of viability and function, and the synthesis of urea and albumin was 1~3 times higher than that of controls.
Conclusions This microcapsule has excellent biological and mechanical properties.This system can be of great benefit for use in bioartificial liver and cell transplantation.