Abstract:Objective：To investigate the changes of Toll-like receptor 2 and 4 gene expression in acute injury liver tissue of acute hemorrhagic necrotizing pancreatitis (AHNP) rats and the efficacy of intervention with chloroquine(CQ). Methods：Seventy SD male rats were randomly divided into Sham-operated group (n=10), AHNP group (n=30) and Chloroquine-treated group (n=30). Levels of serum amylase, ALT, AST, NO and TNF-α were detected. TLR2/4mRNA expression in the livers were measured by RT-PCR. Results： Comparing to sham-operated group, TLR2/4mRNA was markedly increased at 3 hours in liver tissues in AHNP group, peaking at 6～12 hours (P<0.05 ). As liver injuries were aggravated, the levels of serum amylase, ALT, AST and TNF-α were increased and the level of NO in livers was decreased (P<0.05 ). Treatment with CQ could effectively inhibit TLR2/4mRNA expression (P<0.05), and the degree of liver injuries was decreased, and the levels of serum amylase, ALT, AST and TNF-α were decreased and the levels of NO were also markedly increased (P<0.05 ). Conclusions：The expression of TLR2/4mRNA is increased in livers in AHNP. Up-regulation of TLR2/4mRNA expression in livers may be importantly involved in the pathogenesis and development of acute liver injury in AHNP rats. CQ may have protective effect against liver injuries induced by AHNP.