Abstract:Objective:To develop a rapid donor liver harvesting method in orthotopic liver transplantation in rats, and to study the stability and the success rate of the model. Methods:A total of 200 healthy adult male SD rats were randomly divided into two groups: the experimental group (rapid donor liver harvesting transplant group), and the control group (conventional liver transplant group). The operation time, cold ischemic time and warm ischemic time of the donor liver, recipient post-transplation liver function and the success rate of the operation between the 2 grous were cpmpared. Results:In the control group, the operation time of donor harvesting was (33.8±4.2) min，warm ischemic time and cold ischemic time of donor liver was(3.5±1.2) min and (62.0±4.2) min，with a 80% rate of success. The new method reduced the time for donor surgery to (13.1±2.2)min, and reduced the warm ischemic time and cold ischemic time of donor liver to 0 min and (38.0±3.1)min respectively，and with a 94% rate of success(all P<0.05). In addition，recipient liver function recovered more quickly in the experimental group. Conclusions:The novel model of rapid donor liver harvesting in rat orthotopic liver transplantation reduces the operation time, improves the quality of the donor liver, and enhances the success rate of operation, and provides a more scientific model for relevant researches．