Abstract:Objective:To investigate the ischemic injury of hepatic cell caused by hepatic artery occlusion. Methods:The hepatic artery was occluded in 20 dogs via operation, while the portal vein remained patent . Specimens were gained from the right liver at four time points:before occlusion of the hepatic artery , 20 minutes, 40 minutes and 60 minutes after artery occlusion. Each specimen was examined by HE and BCL-2 by immunohistochemistry. The gray scale of BCL-2 in HE sections was detected. Results:Hepatic cellular injury was obvious 20 minutes after occlusion of the hepatic artery. Irreversible hepatic cellular injury was observed 60 minutes after hepatic artery occlusion. The results showed that the gray scale of BCL-2 at every time point after hepatic artery occlusion were significantly different from that before hepatic artery occlusion(P<0.0001). Besides, the hepatic cell injury and BCL-2 expression at 20 minutes and 40minutes after occlusion was significantly different from that of 60 minutes(P<0.0001),respectively. Conclusions:There was obvious hepatic cellular injury 20 minutes after hepatic artery occlusion, and irreversible hepatic cellular injury occured 60 minutes after hepatic artery occlusion.