Abstract:Objective：To study the effects of cinobufacin(cino) combined with fluorouracil(5-FU) on inhibiting proliferation and inducing apoptosis of human gastric carcinoma cells in vitro. Methods：The experiment was divided into control group,cinobufacin group,5-FU group and cino+5-FU group. Cell morphological variation,cell inhibitory rate, cell cycle and ratio of apoptotic cell of human gastric carcinoma cell line BGC-823 were studied by cell culture, inverse microscopy, fluoroscopy, MTT assay and flow cytometry on different concentrations of cino and 5-FU. Results：Cino could markably inhibit proliferation of human gastric carcinoma cells in time-and dose-dependent response. The cino+5-FU group inhibited the rate of proliferation of BGC-823 cells was significantly more than either cino or 5-FU alone group(P<0.05). The quantitative analysis showed the same result. Cino+5-FU can decrease the proportion of G0/G1 phase and increase of S phase in cell cycle. Most of the cells were arrested in the S phase. Conclusions：Cino has the effect of inhibition on the proliferation and induction of apoptosis in human BGC-823 gastric carcinoma cell. Cino combined with 5-FU could enhance the antineoplastic effect of 5-FU, and showed time-and dose-dependent response.