Abstract:Objective：To investigate relationships between single nucleotide polymorphisms(SNPs) of tumor necrosis factor-α(TNFα) or interleukin-6(IL-6) promoter genes and gastric adenocarcinoma with or with out helicobacter pylori(HP) infection. Methods：The SNPs of TNF-α(-238G/A and -308G/A) and IL-6(-597G/A,-174G/C and -572G/C) were determined by gene chip in 130 patients with gastric adenocarcinoma and 142 healthy controls.The sera concentrations of IgG,IgM and IgA of HP antibodies were measured by ELISA in all cases and controls.Results：Pylori infection was detected in 69.2% of 130 patients and 46.5% of 142 controls(P<0.01, odds ratio［OR］=2.59，95% confidence interval ［95%CI］=1.58～3.93). Frequencies of TNF-α-238GA genotype and A allele in patients with gastric adenocarcinoma were significantly higher than those in healthy controls(P<0.01，OR=2.44，95%CI=1.41～4.22 and P<0.01，OR=2.13，95%CI=1.22～3.73,respectively).Frequencies of TNF-α-238GA genotype and A allele in patients with gastric adenocarcinoma with HP infection were significantly higher than those in HP-negtive patients with gastric adenocarcinoma(P<0.05，OR=4.53,95%CI=1.16-17.68 and P<0.01，OR=3.52,95%CI=1.64-7.54,respectively). Frequencies of TNF-α-238GA genotype and A allele in patients with poorly-differentiated gastric adenocarcinoma were significantly higher than those in well-differentiated group(P<0.05，OR=4.16,95%CI=1.21～14.32). The proportion of individuals carrying the IL-6-572CC genotype was significantly lower in the patients with gastric adenocarcinoma than in the controls(P<0.01，OR=0.17，95% CI=0.064～0.465). No association was found between any of the other polymorphisms and patients with gastric adenocarcinoma or patients with gastric adenocarcinoma and HP infection.Conclusions：TNF-α-238GA genotype and A allele were significantly related to patients with gastric adenocarcinoma or patients with gastric adenocarcinoma and HP infection. IL-6-572CC genotype can have a protective function against susceptibility to gastric cancer.